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Spectroscopy > Fluorscence Lifetime

 

 

ISS offers a complete line of spectrofluorometers for steady-state and time-resolved fluorescence applications, time-resolved confocal microscopy, lifetime imaging and fluorescence correlation spectroscopy.

 

Several components allow the user to assemble a custom-built spectrofluorometer tailored to specific applications. An extensive line of fluorescence accessories that can also be used in other commercial spectrofluorometers complements the ISS spectrofluorometers.

 

ChronosBH

ISS technology combined with Becker & Hickl electronics for precise TCSPC measurements with a variety of pulsed light sources.

ChronosBH™ is a fully-automated, user-friendly, time-domain instrument with picosecond resolution based on laser diodes and other pulsed light sources such as white, continuous wave and Ti:Sapphire lasers. The optical design and instrument control of ChronosBH™ are state-of-the-art, and it includes Vinci, a powerful, Windows-based software package.

Time-Domain Fluorescence Lifetime Measurements On ChronosBH™

Features

  • Compact and portable
  • Interchangeable solid state light modules
  • Full hardware automation using Vinci
  • Full acquisition automation using Vinci

Time-Resolved Measurements

  • Single- and multi-exponential intensity decays
  • Anisotropy decays (rotational correlation times)
  • Time-resolved kinetics
  • Time-resolved spectra
  • Time-resolved FRET

 

Chronos™

The affordable solution for picosecond lifetime measurements

The ISS Chronos is the first, frequency-domain fluorescence lifetime instrument based on the use of laser diodes and light emitting diodes (LED) as excitation sources. This powerful tool provides all the benefits of a full lifetime fluorometer at an affordable price. The ISS Chronos has been enhanced for those users operating at standard wavelengths of excitation typically at a set region. The Chronos is a fully-automated instrument using a Windows based software program and can be upgraded to steady-state acquisition.

For the Phase and Demodulation data of indocynine green (ICG) acquired by a laser diode, click here

Chronos, Lifetime fluorescence measurements using laser diodes and LEDs

Features

  • Compact and portable
  • Interchangeable modules for each wavelength
  • Full hardware automation
  • Full acquisition automation
  • Speed ­ measurements from complex decays are acquired in less than one minute

Measurements

  • Lifetime determination of multi-exponential decays
  • Lifetime analysis using continuous distributions
  • Rotational motions of molecules
  • Phase and modulation resolved kinetics
  • Phase and modulation resolved spectra
  • Lifetime millisecond kinetics
  • Time-resolved spectra

 

K2™

Multifrequency Cross-Correlation Phase and Modulation Fluorometer

The K2 family of Multifrequency Cross-Correlation Phase and Modulation Fluorometers introduces a new approach to fluorescence and phosphorescence instrumentation for the research laboratory. Its optical design and automatic instrument control are state-of-the-art for steady-state and dynamic fluorescence measurements. Its full automation and user friendly software make the instrument easy to use for both the student and the scientist. The K2 operates as a steady-state fluorometer for the measurement and analysis of excitation, emission, and polarization (anisotropy) spectra; for synchronous luminescence spectra; and for kinetics studies. The K2 is a multifrequency cross-correlation phase and modulation fluorometer for decay times determinations with picosecond resolution; for the measurement of rotational correlation times of molecules; phase and modulation resolved spectra; and for phase and modulation resolved kinetics lifetime.

Six models of the K2 are available, each fully automated and upgradeable to any other model:

K2-001 K2-Analog
The instrument operates at a fixed 40 Hz cross-correlation frequency and it is capable of modulation frequencies up to 350 MHz.

K2-002 K2-Digital
The cross-correlation is user-selectable up to 5000 Hz and data acquisition is performed through a FFT routine; the instrument covers the modulation frequency range from 300 KHz up to 350 MHz with a lamp and up to 450 MHz using a cw laser.

K2-003 K2-FastScan
The FastScan is our most popular model: lifetime data acquisition on a routine sample can be acquired in less than one minute using either a lamp or a laser.

K2-004 1.2 GHz Upgrade
This model requires a mode-locked laser as excitation source and a microchannel plate detector (MCP-PMT) for the collection of fluorescence. Alternatively, a laser diode can be utilized as the light source up to about 900 MHz. The cross-correlation is user-selectable up to 5000 Hz and data acquisition is performed through a FFT routine. It includes the FastScan upgrade and operates at modulation frequency up to 1.2 GHz.

K2-005 6 GHz Upgrade
The K2-005 is an upgrade of the K2-004: it operates in the frequency range up to 6 GHz.

K2-006 10 GHz Upgrade
The unique feature of K2-006, is the capability to work up to 10 GHz modulation frequency. It requires a mode-locked laser as the excitation source and a MCP-PMT as the light detector. The signal is detected using the super-heterodyning technique: the detected fluorescence signal is first scaled down to an intermediate frequency which, in turn, is heterodyned to the cross-correlation frequency.

 

 

 

 

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