Time-resolved Laser Scanning Confocal Microscopes
Alba STED -FLIM/FFS Laser Scanning Microscope
Stimulated Emission Depletion
Ring Laser & excitation laser
Phasar plot/FILM
Sample Piazo scanning
Confocal volume motorised
Z focus motorised
Nikon Microscope
Choice of excitation lasers
Choice of objectives.
Alba -Time-resolved Confocal Microscope
Single Molecule sensitivity – PMT/APD
Multi photon
ps/ns lasers/LED
LED – ns- 265,280,295,300,320,335,340,345,370,460,
485,520nm
Lasers – ps 370,405,440,473,488,514,635,660,780,830nm
Confocal volume motorised
FCS & FILM
z focus motorised
Image scanning XY Galvo or Piezo stage scanning
Objectives 10x,20x,50x,100x, oil & water
Microscope – Inverted Nikon/Zeiss/Olympus
Q2 Modular Confocal
Microscope
Single Molecule sensitivity – Multiphoton
PMT/APD
Confocal volume – manual or controlled by dia of fiber
FCS & FILM
LED – ns- 265,280,295,300,320,335,340,345,370,460,
485,520nm
Lasers – ps 370,405,440,473,488,514,635,660,780,830nm
Galvo scanning / piezo sample scanning
Nikon/Zeiss/Olympus
Objectives 10x,20x,50x,100x, oil & water
PL1 Material Sciences Confocal Workstation
Confocal microscope – material reflective mode
Single Molecule sensitivity – Multiphoton
PMT/APD
Confocal volume – manual or controlled by dia of fiber
FCS & FILM
LED – ns- 265,280,295,300,320,335,340,345,370,460,
485,520nm
Lasers – ps 370,405,440,473,488,514,635,660,780,830nm
Galvo scanning / piezo sample scanning
Nikon/Zeiss/Olympus
ALBA STED
Stimulated Emission Depletion (STED) is a powerful super resolution microscopy technique that allows for the observation of fluorescence structures with spatial resolution below the diffraction limit.
The Alba STED uses the pulsed excitation and pulsed emission approach (pSTED) to achieve lateral esolution of 60 – 100 nm.
Dynamic Range
Fast Acquisition
